Human TRIM69 Recombinant Protein (N-His) (HP348012)

Name: Human TRIM69 Recombinant Protein (N-His)
Brand: abinScience
SKU: HP348012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HP348012
Description	Recombinant Human TRIM69 Protein, N-His (HP348012) expressed in E. coli, spanning Thr331-Gln500. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q86WT6
Protein length	Thr331-Gln500
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	21.70 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Tripartite motif-containing protein 69, RFP-like domain-containing protein trimless, RNF36, E3 ubiquitin-protein ligase TRIM69, RING finger protein 36, RING-type E3 ubiquitin transferase TRIM69, TRIM69
Background	E3 ubiquitin-protein ligase TRIM69 is a ~57 kDa protein. E3 ubiquitin ligase that plays an important role in antiviral immunity by restricting different viral infections including dengue virus or vesicular stomatitis indiana virus. Ubiquitinates viral proteins such as dengue virus NS3 thereby limiting infection. In addition, acts as a key mediator of type I interferon induced microtubule stabilization by directly associating to microtubules independently of its E3 ligase activity. Also plays a role in cataract formation together with TP53. Mechanistically, inhibits UVB-induced cell apoptosis and reactive oxygen species (ROS) production by inducing TP53 ubiquitination. 1. Han, Y. et al. (2012) Biochemical and biophysical research communications 429, 6-11. PMID: 23131556 2. Wang, K. et al. (2018) PLoS pathogens 14, e1007287. PMID: 30142214 3. Rihn, SJ. et al. (2019) Journal of virology 93. PMID: 31375575 4. Kueck, T. et al. (2019) Journal of virology 93. PMID: 31578292 5. Rong, X. et al. (2019) Redox biology 22, 101157. PMID: 30844644 6. Song, Y. et al. (2022) Proceedings of the National Academy of Sciences of the United States of America 119, e2211467119. PMID: 36251989 7. Wang, Y. et al. (2023) Nucleic acids research 51, 10568-10589. PMID: 37739411
Note	For research use only.

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SDS-PAGE for Recombinant Human TRIM69 Protein, N-His

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Datasheet

Human TRIM69 Recombinant Protein (N-His).pdf

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Human TRIM69 Recombinant Protein (N-His) (HP348012)

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