Recombinant European white birch Bet v 1/Allergen Bet v I-A Protein, N-His (PR095012)

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Datasheet

Overview

Catalog No.	PR095012
Description	Recombinant European white birch Bet v 1/Allergen Bet v I-A Protein, N-His (PR095012) expressed in E. coli, spanning Met1-Asn160. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P15494
Protein length	Met1-Asn160
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Betula pendula (European white birch)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	19.87 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Allergen Bet v I-A, BETVI, BETVIA, Bet v 1-A, Major pollen allergen Bet v 1-A
Background	Major pollen allergen Bet v 1-A (Allergen Bet v I-A) is a ~17 kDa protein. May be a general steroid carrier protein.
Note	For research use only.

Images

SDS-PAGE

SDS-PAGE for Recombinant European white birch Bet v 1/Allergen Bet v I-A Protein, N-His
Bioactivity

Detected by Anti-Bet v 1/Allergen Bet v I-A Antibody (SAA0656) (Cat No.: PR095023) in indirect ELISA.
Western blot

Detected by Anti-Bet v 1 monoclonal antibody (Catalog No: PR095173)
Bioactivity

Detected by Anti-Bet v 1/Allergen Bet v I-A Antibody (H3-1) (Catalog No: PR095173) in indirect ELISA
Bioactivity

Detected by Bremzalerbart (Catalog No: PR095036) in indirect ELISA.
Bioactivity

Detected by Atisnolerbart (Catalog No: PR095026) in indirect ELISA.
Bioactivity

Detected by Umesolerbart (Catalog No: PR095016) in indirect ELISA.
Bioactivity

Detected by SAA0666 (Catalog No: PR095123) in indirect ELISA.
Bioactivity

Detected by SAA0667 (Catalog No: PR095133) in indirect ELISA.
Bioactivity

Detected by SAA0668 (Catalog No: PR095143) in indirect ELISA.
Bioactivity

Detected by SAA0668 (Catalog No: PR095183) in indirect ELISA.