Human TENT4A/PAPD7/TRF4 Recombinant Protein (N-His) (HP255012)

Name: Human TENT4A/PAPD7/TRF4 Recombinant Protein (N-His)
Brand: abinScience
SKU: HP255012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HP255012
Description	Recombinant Human TENT4A/PAPD7/TRF4 Protein, N-His (HP255012) expressed in E. coli, spanning Gly178-Ala536. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q5XG87
Protein length	Gly178-Ala536
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	42.68 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Non-canonical poly(A) RNA polymerase PAPD7, Topoisomerase-related function protein 4-1, Terminal nucleotidyltransferase 4A, LAK-1, TUTase 5, PAP-associated domain-containing protein 7, Terminal uridylyltransferase 5, DNA polymerase sigma, TRF4, TRF4-1, POLS, PAPD7, TENT4A, TRAMP-like complex polyadenylate polymerase, Terminal guanylyltransferase
Background	Terminal nucleotidyltransferase 4A (TENT4A/PAPD7) is a ~84 kDa protein. Terminal nucleotidyltransferase that catalyzes preferentially the transfer of ATP and GTP on RNA 3' poly(A) tail creating a heterogeneous 3' poly(A) tail leading to mRNAs stabilization by protecting mRNAs from active deadenylation. Also functions as a catalytic subunit of a TRAMP-like complex which has a poly(A) RNA polymerase activity and is involved in a post-transcriptional quality control mechanism. Polyadenylation with short oligo(A) tails is required for the degradative activity of the exosome on several of its nuclear RNA substrates. Has no terminal uridylyltransferase activity, and does not play a role in replication-dependent histone mRNA degradation via uridylation. 1. Ogami, K. et al. (2013) Biochemical and biophysical research communications 432, 135-40. PMID: 23376078 2. Lim, J. et al. (2018) Science (New York, N.Y.) 361, 701-704. PMID: 30026317
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human TENT4A/PAPD7/TRF4 Protein, N-His

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Datasheet

Human TENT4A/PAPD7/TRF4 Recombinant Protein (N-His).pdf

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Human TENT4A/PAPD7/TRF4 Recombinant Protein (N-His) (HP255012)

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