Human LTB/TNFC Recombinant Protein (C-His) (HC386011)

Name: Human LTB/TNFC Recombinant Protein (C-His)
Brand: abinScience
SKU: HC386011
Price: 264 USD
Availability: InStock

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Overview

Catalog No.	HC386011
Description	Recombinant Human LTB/TNFC Protein, C-His (HC386011) expressed in Insect Cells, spanning Gly86-Gly244. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	Insect cells
Accession	Q06643
Protein length	Gly86-Gly244
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	21.90 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Lymphotoxin-beta, LT-beta, Tumor necrosis factor C, TNF-C, Tumor necrosis factor ligand superfamily member 3, LTB, TNFC, TNFSF3
Background	Lymphotoxin-beta (LTB/TNFC) is a ~25 kDa protein. Cytokine that binds to LTBR/TNFRSF3. May play a specific role in immune response regulation. Provides the membrane anchor for the attachment of the heterotrimeric complex to the cell surface. Isoform 2 is probably non-functional. 1. Sudhamsu, J. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, 19896-901. PMID: 24248355
Note	For research use only.

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SDS-PAGE for Recombinant Human LTB/TNFC Protein, C-His

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Datasheet

Human LTB/TNFC Recombinant Protein (C-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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C₁ × V₁ = C₂ × V₂

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Human LTB/TNFC Recombinant Protein (C-His) (HC386011)

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