Human CNOT7 Recombinant Protein (N-His) (HA970012)

Name: Human CNOT7 Recombinant Protein (N-His)
Brand: abinScience
SKU: HA970012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HA970012
Description	Recombinant Human CNOT7 Protein, N-His (HA970012) expressed in E. coli, spanning Arg11-Ser285. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q9UIV1
Protein length	Arg11-Ser285
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	34.00 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	CNOT7, BTG1-binding factor 1, CAF-1, CAF1, Caf1a, CCR4-NOT transcription complex subunit 7, CCR4-associated factor 1
Background	CCR4-NOT transcription complex subunit 7 (CNOT7) is a ~32 kDa protein. Has 3'-5' poly(A) exoribonuclease activity for synthetic poly(A) RNA substrate. Its function seems to be partially redundant with that of CNOT8. Catalytic component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. During miRNA-mediated repression the complex also seems to act as translational repressor during translational initiation. Additional complex functions may be a consequence of its influence on mRNA expression. 1. Horiuchi, M. et al. (2009) The Journal of biological chemistry 284, 13244-55. PMID: 19276069 2. Cooke, A. et al. (2010) The Journal of biological chemistry 285, 28506-13. PMID: 20634287 3. Kim, TH. et al. (2019) Science (New York, N.Y.) 365, 825-829. PMID: 31439799 4. Aslam, A. et al. (2009) Molecular biology of the cell 20, 3840-50. PMID: 19605561 5. Piao, X. et al. (2010) Molecular and cellular biology 30, 1486-94. PMID: 20065043 6. Doidge, R. et al. (2012) PloS one 7, e51331. PMID: 23236473
Note	For research use only.

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SDS-PAGE for Recombinant Human CNOT7 Protein, N-His

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Datasheet

Human CNOT7 Recombinant Protein (N-His).pdf

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Human CNOT7 Recombinant Protein (N-His) (HA970012)

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