ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
27.35 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names
Helicase A-binding protein 95, AKAP8L, Neighbor of AKAP95, Neighbor of A-kinase-anchoring protein 95, Homologous to AKAP95 protein, A-kinase anchor protein 8-like, AKAP8-like protein, HA95, NAKAP, NAKAP95, HAP95
Background
A-kinase anchor protein 8-like (AKAP8L) is a ~71 kDa protein. Could play a role in constitutive transport element (CTE)-mediated gene expression by association with DHX9. Increases CTE-dependent nuclear unspliced mRNA export. Proposed to target PRKACA to the nucleus but does not seem to be implicated in the binding of regulatory subunit II of PKA. May be involved in nuclear envelope breakdown and chromatin condensation. May be involved in anchoring nuclear membranes to chromatin in interphase and in releasing membranes from chromating at mitosis.
1. Westberg, C. et al. (2000) The Journal of biological chemistry 275, 21396-401. PMID: 10748171
2. Yang, JP. et al. (2001) The Journal of biological chemistry 276, 30694-700. PMID: 11402034
3. Orstavik, S. et al. (2000) Biology of the cell 92, 27-37. PMID: 10761695
4. Han, I. et al. (2002) Molecular and cellular biology 22, 2136-46. PMID: 11884601
5. Martins, SB. et al. (2000) Journal of cell science 113 Pt 21, 3703-13. PMID: 11034899
6. Martins, S. et al. (2003) The Journal of cell biology 160, 177-88. PMID: 12538639
7. Li, Y. et al. (2006) Genes & development 20, 2566-79. PMID: 16980585
8. Kvissel, AK. et al. (2007) Experimental cell research 313, 2795-809. PMID: 17594903
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Recombinant Human AKAP8L Protein, N-His
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