Bacillus licheniformis BliGO/thiO Recombinant Protein (N-GST & C-His) (JN872022)

Name: Bacillus licheniformis BliGO/thiO Recombinant Protein (N-GST & C-His)
Brand: abinScience
SKU: JN872022
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	JN872022
Description	Recombinant Bacillus licheniformis BliGO/thiO Protein, N-GST & C-His (JN872022) expressed in E. coli, spanning Met1-Arg369. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	S5FMM4
Protein length	Met1-Arg369
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Bacillus licheniformis
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	67.09 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Glycine oxidase, GO, 1.4.3.19, BliGO, thiO
Background	Glycine oxidase (BliGO/thiO) is a ~40 kDa protein. Catalyzes the FAD-dependent oxidative deamination of glycine, leading to glyoxylate, ammonia and hydrogen peroxide. Is also able to act on various amines and D-amino acids to yield the corresponding alpha-keto acids, ammonia/amine, and hydrogen peroxide. Can also oxidize the herbicide glyphosate (N-phosphonomethylglycine), and thus may be involved in the degradation pathway that allows B.licheniformis J33-8 to grow with glyphosate as the sole source of carbon. Is essential for thiamine biosynthesis since the oxidation of glycine catalyzed by ThiO generates the glycine imine intermediate (dehydroglycine) required for the biosynthesis of the thiazole ring of thiamine pyrophosphate. 1. Zhang, K. et al. (2016) Enzyme and microbial technology 85, 12-8. PMID: 26920475
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Bacillus licheniformis BliGO/thiO Protein, N-GST & C-His

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Datasheet

Bacillus licheniformis BliGO/thiO Recombinant Protein (N-GST & C-His).pdf

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Bacillus licheniformis BliGO/thiO Recombinant Protein (N-GST & C-His) (JN872022)

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Datasheet

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