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Recombinant Rabies virus/RABV G/Glycoprotein Protein, C-10*His (VK584021)

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Recombinant Rabies virus/RABV G/Glycoprotein Protein, C-10*His

Recombinant Rabies virus/RABV G/Glycoprotein Protein, C-10*His

Recombinant Rabies virus/RABV G/Glycoprotein Protein, C-10*His

Recombinant Rabies virus/RABV G/Glycoprotein Protein, C-10*His

Recombinant Rabies virus/RABV G/Glycoprotein Protein, C-10*His

Recombinant Rabies virus/RABV G/Glycoprotein Protein, C-10*His

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100ug
1mg

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Overview
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References
Datasheet

Overview

Catalog No.	VK584021
Description	*Recombinant Rabies virus/RABV G/Glycoprotein Protein, C-10His (VK584021) expressed in Mammalian Cells, spanning Met1-Tyr459. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●Mammalian Expression — Proper folding and native glycosylation. ●High Purity** — >90% purity verified by SDS-PAGE.
Expression system	Mammalian Cells
Accession	P08667
Protein length	Met1-Tyr459
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Rabies virus (strain Pasteur vaccins / PV) (RABV)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	52.96 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Glycoprotein, G
Background	Glycoprotein is a ~58 kDa protein. Attaches the virus to host cellular receptor, inducing endocytosis of the virion by using different host proteins including TFRC, GRM2 and ITGB1. In the endosome, the acidic pH induces conformational changes in the glycoprotein trimer, which trigger fusion between virus and cell membrane. There is convincing in vitro evidence that the muscular form of the nicotinic acetylcholine receptor (nAChR), the neuronal cell adhesion molecule (NCAM), and the p75 neurotrophin receptor (p75NTR) bind glycoprotein and thereby facilitate rabies virus entry into cells. 1. Wang, J. et al. (2018) PLoS pathogens 14, e1007189. PMID: 30028877 2. Shuai, L. et al. (2020) Journal of virology 94. PMID: 31666383 3. Wang, X. et al. (2023) Journal of virology 97, e0161222. PMID: 36779762
Note	For research use only.

Images

SDS-PAGE

SDS-PAGE for Recombinant Rabies virus/RABV G/Glycoprotein Protein, C-10*His
Bioactivity

Detected by Anti-RABV G/Glycoprotein Antibody (RVC58) (Catalog No: VK632063) in indirect ELISA
Bioactivity

Detected by Anti-RABV G/Glycoprotein Antibody (SO57) (Catalog No: VK632113) in indirect ELISA

References

VK584021-SDS-PAGE-1.jpg

Datasheet

Recombinant Rabies virus/RABV G/Glycoprotein Protein, C-10*His.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

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Concentration

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Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

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V₁ (Stock Vol.)

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Working Solution

C₂ (Working Conc.)

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V₂ (Working Vol.)

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Recombinant Rabies virus/RABV G/Glycoprotein Protein, C-10*His