HCMV/HHV-5 UL123/IE1 Recombinant Protein (N-GST & C-His) (VK663012)

Name: HCMV/HHV-5 UL123/IE1 Recombinant Protein (N-GST & C-His)
Brand: abinScience
SKU: VK663012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	VK663012
Description	Recombinant HCMV/HHV-5 UL123/IE1 Protein, N-GST & C-His (VK663012) expressed in E. coli, spanning Asp12-Glu382. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P13202, P03169, F5HCM1
Protein length	Asp12-Glu382
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Human cytomegalovirus (HCMV) (HHV-5) (Human herpesvirus 5)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	70.60 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	regulatory protein IE1, UL123
Background	Immediate early protein IE1 (UL123) is a ~55 kDa protein. Plays an important role in transactivating viral early genes as well as activating its own promoter, probably by altering the viral chromatin structure. Expression of IE1 and IE2 proteins is critical for the establishment of lytic infection and reactivation from viral latency. Disrupts PML-associated ND10 nuclear bodies by interfering with host PML and SP100 sumoylation thereby altering the regulation of type I and type II interferon-induced gene expression. Promotes efficient viral growth by interacting with and directing host SP100 to degradation, leading to enhanced acetylation level of histones. In addition, functions in counteracting the host innate antiviral response. 1. Arend, KC. et al. (2016) Journal of virology 90, 8855-65. PMID: 27466417 2. Schilling, EM. et al. (2017) Journal of virology 91. PMID: 27903803 3. Schweininger, J. et al. (2021) PLoS pathogens 17, e1009863. PMID: 34370791 4. Reitsma, JM. et al. (2013) Journal of virology 87, 10763-76. PMID: 23903834
Note	For research use only.

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SDS-PAGE for Recombinant HCMV/HHV-5 UL123/IE1 Protein, N-GST & C-His

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Datasheet

HCMV/HHV-5 UL123/IE1 Recombinant Protein (N-GST & C-His).pdf

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HCMV/HHV-5 UL123/IE1 Recombinant Protein (N-GST & C-His) (VK663012)

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