EV71 P2A/Protease 2A Recombinant Protein (N-His) (VK633022)

Name: EV71 P2A/Protease 2A Recombinant Protein (N-His)
Brand: abinScience
SKU: VK633022
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	VK633022
Description	Recombinant EV71 P2A/Protease 2A Protein, N-His (VK633022) expressed in E. coli, spanning Gly863-Gln1012. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q66479
Protein length	Gly863-Gln1012
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Human enterovirus 71 (strain 7423/MS/87) (EV71) (EV-71)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	18.75 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Genome polyprotein, Protease 2A, P2A, 3.4.22.29, Picornain 2A, Protein 2A
Background	Genome polyprotein (P2A/Protease 2A) is a ~242 kDa protein. Forms an icosahedral capsid of pseudo T=3 symmetry with capsid proteins VP2 and VP3. The capsid is 300 Angstroms in diameter, composed of 60 copies of each capsid protein and enclosing the viral positive strand RNA genome. Capsid protein VP1 mainly forms the vertices of the capsid. Capsid protein VP1, together with VP2, interacts with host cell receptor SCARB2 to provide virion attachment to target host cells. This attachment induces virion internalization. 1. Zhou, D. et al. (2019) Nature microbiology 4, 414-419. PMID: 30531980
Note	For research use only.

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SDS-PAGE for Recombinant EV71 P2A/Protease 2A Protein, N-His

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Datasheet

EV71 P2A/Protease 2A Recombinant Protein (N-His).pdf

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EV71 P2A/Protease 2A Recombinant Protein (N-His) (VK633022)

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