EV71 VP1/P1D Recombinant Protein (N-Fc) (VK633042)

Name: EV71 VP1/P1D Recombinant Protein (N-Fc)
Brand: abinScience
SKU: VK633042
Price: 298 USD
Availability: InStock

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Overview

Catalog No.	VK633042
Description	Recombinant EV71 VP1/P1D Protein, N-Fc (VK633042) expressed in E. coli, spanning Gly566-Leu862. Purity: >90% by SDS-PAGE. Highlights ●Fc Fusion — Enhanced stability with Protein A/G purification compatibility. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	Mammalian cells
Accession	F5AU45
Protein length	Gly566-Leu862
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Enterovirus A (GZ2010-156)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	60.26 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Genome polyprotein, Capsid protein VP1, P1D, Virion protein 1
Background	Genome polyprotein (VP1/P1D) is a ~242 kDa protein. Acts as a primer for viral RNA replication and remains covalently bound to viral genomic RNA. VPg is uridylylated prior to priming replication into VPg-pUpU. The oriI viral genomic sequence may act as a template for this. The VPg-pUpU is then used as primer on the genomic RNA poly(A) by the RNA-dependent RNA polymerase to replicate the viral genome. During genome replication, the VPg-RNA linkage is removed by the host TDP2, thereby accelerating replication.
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant EV71 VP1/P1D Protein, N-Fc

References

Datasheet

EV71 VP1/P1D Recombinant Protein (N-Fc).pdf

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Formula

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EV71 VP1/P1D Recombinant Protein (N-Fc) (VK633042)

Overview

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Datasheet

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