Arabidopsis rbcL Recombinant Protein (N-His) (PR005012)

Name: Arabidopsis rbcL Recombinant Protein (N-His)
Brand: abinScience
SKU: PR005012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	PR005012
Description	Recombinant Arabidopsis rbcL Protein, N-His (PR005012) expressed in E. coli, spanning Met1-Glu479. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	O03042
Protein length	Met1-Glu479
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Arabidopsis thaliana (Mouse-ear cress)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	55.12 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Ribulose bisphosphate carboxylase large chain, RuBisCO large subunit, EC:4.1.1.39, rbcL
Background	Ribulose bisphosphate carboxylase large chain is a ~52 kDa protein. RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in the photorespiration process (Probable). Both reactions occur simultaneously and in competition at the same active site (Probable). Binds to abscisic acid (ABA). 1. Galka, MM. et al. (2015) PloS one 10, e0133033. PMID: 26197050
Note	For research use only

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SDS-PAGE for Recombinant Arabidopsis rbcL Protein, N-His

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Datasheet

Arabidopsis rbcL Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Arabidopsis rbcL Recombinant Protein (N-His) (PR005012)

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