Human RNF126 Recombinant Protein (N-His) (HC013012)

Name: Human RNF126 Recombinant Protein (N-His)
Brand: abinScience
SKU: HC013012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HC013012
Description	Recombinant Human RNF126 Protein, N-His (HC013012) expressed in E. coli, spanning Met1-Ser311. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q9BV68
Protein length	Met1-Ser311
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	36.02 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	E3 ubiquitin-protein ligase RNF126, EC:2.3.2.27, RING finger protein 126, RNF126
Background	E3 ubiquitin-protein ligase RNF126 is a ~33 kDa protein. E3 ubiquitin-protein ligase that mediates ubiquitination oF target proteins. Depending on the associated E2 ligase, mediates 'Lys-27'-, 'Lys-29'-, 'Lys-48'- and/or 'Lys-63'-linked polyubiquitination of substrates. Part of a BAG6-dependent quality control process ensuring that proteins of the secretory pathway that are mislocalized to the cytosol are degraded by the proteasome. Probably acts by providing the ubiquitin ligase activity associated with the BAG6 complex and be responsible for ubiquitination of the hydrophobic mislocalized proteins and their targeting to the proteasome. May also play a role in the endosomal recycling of IGF2R, the cation-independent mannose-6-phosphate receptor. 1. Delker, RK. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, 1029-34. PMID: 23277564 2. Smith, CJ. et al. (2014) Experimental cell research 320, 219-32. PMID: 24275455 3. Rodrigo-Brenni, MC. et al. (2014) Molecular cell 55, 227-37. PMID: 24981174 4. Liu, W. et al. (2023) Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10, e2203884. PMID: 36563124 5. Mock, JY. et al. (2017) Proceedings of the National Academy of Sciences of the United States of America 114, 11679-11684. PMID: 29042515 6. Smith, CJ. et al. (2013) Journal of cell science 126, 1366-80. PMID: 23418353 7. Zhi, X. et al. (2013) Cancer research 73, 385-94. PMID: 23026136
Note	For research use only

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SDS-PAGE for Recombinant Human RNF126 Protein, N-His

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Datasheet

Human RNF126 Recombinant Protein (N-His).pdf

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Human RNF126 Recombinant Protein (N-His) (HC013012)

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