Human RPA1 Recombinant Protein (N-His) (HA686012)

Name: Human RPA1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HA686012
Price: 211 USD
Availability: InStock

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Catalog No.	HA686012
Description	Recombinant Human RPA1 Protein, N-His (HA686012) expressed in E. coli, spanning Ile187-Met616. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P27694
Protein length	Ile187-Met616
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	51.04 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Replication protein A 70 kDa DNA-binding subunit; RP-A p70; Replication factor A protein 1; RF-A protein 1; Single-stranded DNA-binding protein; Replication protein A 70 kDa DNA-binding subunit, N-terminally processed; RPA1; REPA1; RPA70
Background	Replication protein A 70 kDa DNA-binding subunit is a ~68 kDa protein. As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. 1. Gupta, R. et al. (2007) Blood 110, 2390-8. PMID: 17596542 2. Haahr, P. et al. (2016) Nature cell biology 18, 1196-1207. PMID: 27723717 3. Bass, TE. et al. (2016) Nature cell biology 18, 1185-1195. PMID: 27723720 4. Lin, YL. et al. (1998) The Journal of biological chemistry 273, 1453-61. PMID: 9430682 5. Maréchal, A. et al. (2014) Molecular cell 53, 235-246. PMID: 24332808 6. Sleeth, KM. et al. (2007) Journal of molecular biology 373, 38-47. PMID: 17765923 7. Aboussekhra, A. et al. (1995) Cell 80, 859-68. PMID: 7697716 8. DeMott, MS. et al. (1998) The Journal of biological chemistry 273, 27492-8. PMID: 9765279 9. Grudic, A. et al. (2007) Nucleic acids research 35, 7267-78. PMID: 17959650 10. Sharma, R. et al. (2022) Blood 139, 1039-1051. PMID: 34767620
Note	For research use only

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SDS-PAGE for Recombinant Human RPA1 Protein, N-His

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Datasheet

Human RPA1 Recombinant Protein (N-His).pdf

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Human RPA1 Recombinant Protein (N-His) (HA686012)

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