HSV1/HHV-1 ICP0 Recombinant Protein (N-His) (VK083012)

Name: HSV1/HHV-1 ICP0 Recombinant Protein (N-His)
Brand: abinScience
SKU: VK083012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	VK083012
Description	Recombinant HSV1/HHV-1 ICP0 Protein, N-His (VK083012) expressed in E. coli, spanning Leu639-Ser762. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P08393
Protein length	Leu639-Ser762
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Human herpesvirus 1 (HHV-1) (Human herpes simplex virus 1) (HSV-1)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	15.84 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	E3 ubiquitin-protein ligase ICP0, EC:2.3.2.27, Alpha-0 protein, Immediate-early protein IE110, RING-type E3 ubiquitin transferase ICP0, Trans-acting transcriptional protein ICP0, VMW110, ICP0
Background	E3 ubiquitin-protein ligase ICP0 is a ~78 kDa protein. SUMO-targeted ubiquitin ligase that plays an essential role in nuclear antiviral defense evasion triggered by dsDNA viruses. Acts during the initial stages of lytic infection and the reactivation of latent viral genome. Prevents the antiviral effect of nuclear bodies by degrading host PML, SP100, MORC2 and MORC3. Prevents antiviral response to viral DNA induced by IFI16 by degrading it. Additionally, inhibits host IRF3 nuclear signaling to prevent interferon production by the infected cells. 1. Hembram, DSS. et al. (2020) Journal of molecular biology 432, 1952-1977. PMID: 32001251 2. Sloan, E. et al. (2016) Journal of virology 90, 8621-33. PMID: 27440897 3. Gaidt, MM. et al. (2021) Nature 600, 138-142. PMID: 34759314 4. Roubille, S. et al. (2024) Proceedings of the National Academy of Sciences of the United States of America 121, e2412258121. PMID: 39589886
Note	For research use only

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SDS-PAGE for Recombinant HSV1/HHV-1 ICP0 Protein, N-His

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Datasheet

HSV1/HHV-1 ICP0 Recombinant Protein (N-His).pdf

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HSV1/HHV-1 ICP0 Recombinant Protein (N-His) (VK083012)

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