Recombinant SARS-CoV-2 RBD (NB.1.8.1) Protein, C-His (VK565471)

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Overview

Catalog No.	VK565471
Description	Recombinant SARS-CoV-2 RBD (NB.1.8.1) Protein, C-His (VK565471) expressed in Mammalian Cells. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●Mammalian Expression — Proper folding and native glycosylation. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	Mammalian Cells
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Severe acute respiratory syndrome coronavirus 2
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	27.64 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	surface glycoprotein, partial [Severe acute respiratory syndrome coronavirus 2], S, GISAID_ID: EPI_ISL_19029461, N-terminal domain of the S1 subunit of the Spike (S) protein from Severe acute respiratory syndrome coronavirus and related betacoronaviruses in the B lineage; cd21624, trimer interface [polypeptide binding], receptor-binding domain of the S1 subunit of coronavirus spike (S) proteins; cl40478, trimer interface [polypeptide binding], putative receptor binding site [polypeptide binding], receptor binding motif, S1/S2 cleavage region and the S2 fusion subunit of coronavirus spike (S) proteins; cl40439, S1/S2 cleavage region, fusion peptide, internal fusion peptide, heptad repeat 1 [structural motif]
Note	For research use only

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SDS-PAGE

SDS-PAGE for Recombinant SARS-CoV-2 RBD (NB.1.8.1) Protein, C-His

References

Datasheet

Recombinant SARS-CoV-2 RBD (NB.1.8.1) Protein, C-His.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

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Working Solution

C₂ (Working Conc.)

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Recombinant SARS-CoV-2 RBD (NB.1.8.1) Protein, C-His (VK565471)

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Datasheet

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