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Human SLC5A5 Recombinant Protein (N-His) (HD133012)

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Overview
Catalog No.HD133012
Description
Recombinant Human SLC5A5 Protein, N-His (HD133012) expressed in E. coli. Purity: >90% by SDS-PAGE.
Highlights
  • His-Tagged — N-terminal 6×His tag for IMAC purification.
  • E. coli Expression — High-yield, cost-effective production.
  • High Purity — >90% purity verified by SDS-PAGE.
Expression systemE. coli
AccessionQ92911
Protein lengthVal305-Pro340&Pro467-Ile526&Thr550-Leu643
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
SpeciesHomo sapiens (Human)
Nature Recombinant
Endotoxin level Please contact with the lab for this information.
Purity >90% as determined by SDS-PAGE.
Predicted molecular weight 22.90 kDa
Form Lyophilized
Storage buffer Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesNIS, Na(+)/I(-) cotransporter, Na(+)/I(-) symporter, Natrium iodide transporter, SLC5A5, Sodium-iodide symporter, Sodium/iodide cotransporter, Solute carrier family 5 member 5
Background

Sodium/iodide cotransporter is a ~68 kDa protein. Sodium:iodide symporter that mediates the transport of iodide into the thyroid gland. Can also mediate the transport of chlorate, thiocynate, nitrate and selenocynate.

1. Van Sande, J. et al. (2003) Endocrinology 144, 247-52. PMID: 12488351
2. Dayem, M. et al. (2008) The Journal of endocrinology 197, 95-109. PMID: 18372236
3. Wu, SL. et al. (2008) The Journal of endocrinology 199, 213-9. PMID: 18708479
4. Huc-Brandt, S. et al. (2011) Biochimica et biophysica acta 1808, 65-77. PMID: 20797386
5. Thompson, RJ. et al. (2019) Thyroid : official journal of the American Thyroid Association 29, 1485-1498. PMID: 31310151
6. Concilio, SC. et al. (2020) PloS one 15, e0229085. PMID: 32084174
7. Smanik, PA. et al. (1996) Biochemical and biophysical research communications 226, 339-45. PMID: 8806637
8. Saito, T. et al. (1997) The Journal of clinical endocrinology and metabolism 82, 3331-6. PMID: 9329364
Note For research use only
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Formula
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