Human TMEM175 Recombinant Protein (N-GST & C-His) (HD816012)

Name: Human TMEM175 Recombinant Protein (N-GST & C-His)
Brand: abinScience
SKU: HD816012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HD816012
Description	Recombinant Human TMEM175 Protein, N-GST & C-His (HD816012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE.. Highlights ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% as determined by SDS-PAGE.
Expression system	E. coli
Accession	Q9BSA9
Protein length	Ser2-Ser33&Ala161-His184&Arg231-Ser257&Ala397-His416&Leu470-Cys504
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Endosomal/lysosomal proton channel TMEM175, Potassium channel TMEM175, TMEM175, Transmembrane protein 175, hTMEM175
Background	Endosomal/lysosomal proton channel TMEM175 is a ~55 kDa protein. Proton-activated proton channel that catalyzes proton efflux from endosomes and lysosomes to maintain a steady-state pH. Activated at low pH (under pH 4.6) by luminal side protons: selectively mediates lysosomal proton release from lysosomes, eliciting a proton leak that balances V-ATPase activity to maintain pH homeostasis. Regulation of lumenal pH stability is required for autophagosome-lysosome fusion. Also acts as a potassium channel at higher pH, regulating potassium conductance in endosomes and lysosomes. Constitutes the pore-forming subunit of the lysoK(GF) complex, a complex activated by extracellular growth factors. 1. Zheng, W. et al. (2022) Science advances 8, eabm1568. PMID: 35333573 2. Hu, M. et al. (2022) Cell 185, 2292-2308.e20. PMID: 35750034 3. Zhang, J. et al. (2023) Molecular cell 83, 2524-2539.e7. PMID: 37390818 4. Cang, C. et al. (2015) Cell 162, 1101-12. PMID: 26317472 5. Brunner, JD. et al. (2020) eLife 9. PMID: 32267231 6. Lee, C. et al. (2017) Nature 547, 472-475. PMID: 28723891 7. Oh, S. et al. (2020) eLife 9. PMID: 32228865
Note	For research use only

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Datasheet

Human TMEM175 Recombinant Protein (N-GST & C-His).pdf

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Human TMEM175 Recombinant Protein (N-GST & C-His) (HD816012)

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