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Mouse LGALS9 Recombinant Protein (N-His) (MT173012)

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Overview
Catalog No.MT173012
Description
Recombinant Mouse LGALS9 Protein, N-His (MT173012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
  • E. coli Expression — High-yield, cost-effective production.
  • High Purity — >90% as determined by SDS-PAGE.
Expression systemE. coli
AccessionO08573
Protein lengthMet1-Ser149
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
SpeciesMus musculus (Mouse)
Nature Recombinant
Endotoxin level Please contact with the lab for this information.
Purity >90% as determined by SDS-PAGE.
Form Lyophilized
Storage buffer Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesGal-9, Galectin-9, Lgals9
Background

Galectin-9 is a ~40 kDa protein. Binds galactosides. Has high affinity for the Forssman pentasaccharide. Ligand for HAVCR2/TIM3. Binding to HAVCR2 induces T-helper type 1 lymphocyte (Th1) death. Also stimulates bactericidal activity in infected macrophages by causing macrophage activation and IL1B secretion which restricts intracellular bacterial growth.

1. Jayaraman, P. et al. (2010) The Journal of experimental medicine 207, 2343-54. PMID: 20937702
2. Bi, S. et al. (2011) Proceedings of the National Academy of Sciences of the United States of America 108, 10650-5. PMID: 21670307
3. Wu, C. et al. (2014) Immunity 41, 270-82. PMID: 25065622
4. Golden-Mason, L. et al. (2013) Journal of virology 87, 4835-45. PMID: 23408620
5. Steelman, AJ. et al. (2014) Journal of neuroinflammation 11, 144. PMID: 25158758
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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