M. tuberculosis Amidase Rv3717 Recombinant Protein (N-His) (JN179032)

Name: M. tuberculosis Amidase Rv3717 Recombinant Protein (N-His)
Brand: abinScience
SKU: JN179032
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	JN179032
Description	Recombinant M. tuberculosis Amidase Rv3717 Protein, N-His (JN179032) expressed in E. coli. Purity: >90% as determined by SDS-PAGE.. Highlights ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% as determined by SDS-PAGE.
Expression system	E. coli
Accession	I6Y4D2
Protein length	Ala37-Arg253
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Mycobacterium tuberculosis
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	EC:3.5.1.28, N-acetylmuramoyl-L-alanine amidase Rv3717, Zinc-dependent peptidoglycan amidase
Background	N-acetylmuramoyl-L-alanine amidase Rv3717 is a ~24 kDa protein. Cell-wall hydrolase that hydrolyzes the amide bond between N-acetylmuramic acid and L-alanine in cell-wall glycopeptides. Is able to hydrolyze the cell walls of several bacterial species (i.e. Paenibacillus sp., B.avium, E.coli DH5alpha, E.aerogenes, L.acidophilus, B.thuringiensis, B.pumilus, B.subtilis and E.coli W3110), thereby showing that it is a cell-wall hydrolase with broad-spectrum activity. May have a role in peptidoglycan fragment recycling. 1. Prigozhin, DM. et al. (2013) The Journal of biological chemistry 288, 31549-55. PMID: 24019530 2. Kumar, A. et al. (2013) Acta crystallographica. Section D, Biological crystallography 69, 2543-54. PMID: 24311595
Note	For research use only

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M. tuberculosis Amidase Rv3717 Recombinant Protein (N-His).pdf

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M. tuberculosis Amidase Rv3717 Recombinant Protein (N-His) (JN179032)

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