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GMP Grade Human GDF-2/BMP-9 (Tag Free) (HV096011)

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Overview
Catalog No.HV096011
Description
GMP Grade Human GDF-2/BMP-9 (Tag Free) (HV096011) expressed in Mammalian cells. Purity: >95% as determined by SDS-PAGE. by SDS-PAGE.
Highlights
  • ●Recombinant — Consistent lot-to-lot performance.
  • ●High Purity — >95% as determined by SDS-PAGE. by SDS-PAGE for demanding downstream applications.
  • ●Bioactive — Verified biological activity.
Expression systemMammalian cells
AccessionQ9UK05
Protein lengthSer320-Arg429
ApplicationsELISA, Functional assay
SpeciesHomo sapiens (Human)
Biological activity Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells. The expected ED50 for this effect is <2.0 ng/ml.
Nature Recombinant
Endotoxin level <0.01 EU/ug
Purity >95% as determined by SDS-PAGE.
Predicted molecular weight 12.1 kDa
Form Lyophilized
Storage buffer Lyophilized from a 0.22 μm-filtered solution containing 0.085% TFA, 30% ACN, 5% Mannitol, pH 2.5

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution It is recommended to redissolve in 4 mM HCl
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesBMP-9, BMP9, Bone morphogenetic protein 9, GDF-2, GDF2, Growth/differentiation factor 2
Background

Growth/differentiation factor 2 (GDF2/BMP9) is a ~47 kDa protein. Potent circulating inhibitor of angiogenesis. Signals through the type I activin receptor ACVRL1 but not other Alks. Signaling through SMAD1 in endothelial cells requires TGF-beta coreceptor endoglin/ENG.

Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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