GMP Grade Human BMP-2 (Tag Free) (HY576022)

Name: GMP Grade Human BMP-2 (Tag Free)
Brand: abinScience
SKU: HY576022
Price: 420 USD
Availability: InStock

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Overview

Catalog No.	HY576022
Description	Human BMP-2 Recombinant Protein (Tag Free) (HY576022) expressed in E. coli. Purity: >95% as determined by SDS-PAGE. by SDS-PAGE. Highlights ●Recombinant — Consistent lot-to-lot performance. ●High Purity — >95% as determined by SDS-PAGE. by SDS-PAGE for demanding downstream applications. ●Bioactive — Verified biological activity.
Expression system	E. coli
Accession	P12643
Protein length	Gln283-Arg396
Applications	ELISA, Functional assay
Species	Homo sapiens (Human)
Biological activity	Measured alkaline phosphatase production by ATDC-5 chondrogenic cells. The ED50 is ≤0.
Nature	Recombinant
Endotoxin level	<0.01 EU/ug
Purity	>95% as determined by SDS-PAGE.
Predicted molecular weight	12.9 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a 0.22 μm-filtered solution containing 100 mM Acetic acid, 5% Trehalose, pH 3.0 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	It is recommended to redissolve in 4 mM HCl
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	BMP-2, BMP-2A, BMP2, BMP2A, Bone morphogenetic protein 2, Bone morphogenetic protein 2A
Background	Bone morphogenetic protein 2 (BMP2) is a ~44 kDa protein. Growth factor of the TGF-beta superfamily that plays essential roles in many developmental processes, including cardiogenesis, neurogenesis, and osteogenesis. Induces cartilage and bone formation. Initiates the canonical BMP signaling cascade by associating with type I receptor BMPR1A and type II receptor BMPR2. Once all three components are bound together in a complex at the cell surface, BMPR2 phosphorylates and activates BMPR1A. In turn, BMPR1A propagates signal by phosphorylating SMAD1/5/8 that travel to the nucleus and act as activators and repressors of transcription of target genes. 1. Lavery, K. et al. (2008) The Journal of biological chemistry 283, 20948-58. PMID: 18436533 2. Tanaka, K. et al. (2014) Calcified tissue international 94, 454-64. PMID: 24362451 3. Seeherman, HJ. et al. (2019) Science translational medicine 11. PMID: 31019025 4. Wozney, JM. et al. (1988) Science (New York, N.Y.) 242, 1528-34. PMID: 3201241 5. Keller, S. et al. (2004) Nature structural & molecular biology 11, 481-8. PMID: 15064755 6. Weber, D. et al. (2007) BMC structural biology 7, 6. PMID: 17295905 7. Liu, F. et al. (1995) Molecular and cellular biology 15, 3479-86. PMID: 7791754 8. Wang, CY. et al. (2020) Blood 135, 453-456. PMID: 31800957 9. Tsukahara, S. et al. (2006) The Biochemical journal 398, 595-603. PMID: 16771708
Note	For research use only.

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Datasheet

GMP Grade Human BMP-2 (Tag Free).pdf

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GMP Grade Human BMP-2 (Tag Free) (HY576022)

Overview

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References

Datasheet

SDS