GMP Grade Human IFN-gamma (Tag Free) (HF813011)

Name: GMP Grade Human IFN-gamma (Tag Free)
Brand: abinScience
SKU: HF813011
Price: 180 USD
Availability: InStock

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100ug
1mg

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Overview

Catalog No.	HF813011
Description	GMP Grade Human IFN-gamma (Tag Free) (HF813011) expressed in Mammalian cells. Purity: >95% as determined by SDS-PAGE. by SDS-PAGE. Highlights ●Recombinant — Consistent lot-to-lot performance. ●High Purity — >95% as determined by SDS-PAGE. by SDS-PAGE for demanding downstream applications. ●Bioactive — Verified biological activity.
Expression system	Mammalian cells
Accession	P01579
Protein length	Gln24-Gln166
Applications	ELISA, Functional assay
Species	Homo sapiens (Human)
Biological activity	Measured in a cell proliferation assay using Mo7e human leukemia cell line . The ED50 for this effect is ≤ 2 ng/mL.
Nature	Recombinant
Endotoxin level	<0.01 EU/ug
Purity	>95% as determined by SDS-PAGE.
Predicted molecular weight	16.8 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a 0.22 μm-filtered solution containing PBS, 5% Mannitol, 0.01% Tween 80, pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	IFN-gamma, IFNG, Immune interferon, Interferon gamma
Background	Interferon gamma (Interferon gamma,IFN-gamma,Immune interferon,IFNG) is a ~19 kDa protein. Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation. Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription. Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits. 1. El Bougrini, J. et al. (2006) Cellular and molecular biology (Noisy-le-Grand, France) 52, 9-15. PMID: 16914093 2. Hisamatsu, H. et al. (1996) The Journal of experimental medicine 183, 1807-16. PMID: 8666937 3. Greenlund, AC. et al. (1993) The Journal of biological chemistry 268, 18103-10. PMID: 8349687 4. Akiyama, K. et al. (1994) FEBS letters 343, 85-8. PMID: 8163024 5. Fabunmi, RP. et al. (2001) Journal of cell science 114, 29-36. PMID: 11112687 6. Lah, TT. et al. (1995) FEBS letters 363, 85-9. PMID: 7729559
Note	For research use only.

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Datasheet

GMP Grade Human IFN-gamma (Tag Free).pdf

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Formula

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Recombinant Proteins

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GMP Grade Human IFN-gamma (Tag Free) (HF813011)

Overview

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References

Datasheet

SDS