Arabidopsis thalian CUL2 Recombinant Protein (N-His) (PR022012)

Name: Arabidopsis thalian CUL2 Recombinant Protein (N-His)
Brand: abinScience
SKU: PR022012
Price: 422 USD
Availability: InStock

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Catalog No.	PR022012
Description	Arabidopsis thalian CUL2 Recombinant Protein (N-His) (PR022012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE. by SDS-PAGE. Highlights ●Recombinant — Consistent lot-to-lot performance. ●High Purity — >90% as determined by SDS-PAGE. by SDS-PAGE for demanding downstream applications.
Expression system	E. coli
Accession	Q9SRZ0
Protein length	Met1-Leu624
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Arabidopsis thaliana (Mouse-ear cress)
Nature	Recombinant
Endotoxin level	Remove endotoxin and sterilization is required for cell assay.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	74.06 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	AtCUL1, CUL2, Cullin-2
Background	Cullin-2 (CUL2) is a ~86 kDa protein. Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. CUL2 serves as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the E2 ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF). 1. Kamura, T. et al. (2001) The Journal of biological chemistry 276, 29748-53. PMID: 11384984 2. Lin, HC. et al. (2015) Science (New York, N.Y.) 349, 91-5. PMID: 26138980 3. Lin, HC. et al. (2018) Molecular cell 70, 602-613.e3. PMID: 29775578 4. Koren, I. et al. (2018) Cell 173, 1622-1635.e14. PMID: 29779948 5. Zhao, S. et al. (2023) Proceedings of the National Academy of Sciences of the United States of America 120, e2308870120. PMID: 37844242 6. Liwocha, J. et al. (2024) Nature structural & molecular biology 31, 378-389. PMID: 38326650 7. Kamura, T. et al. (2000) Proceedings of the National Academy of Sciences of the United States of America 97, 10430-5. PMID: 10973499 8. Min, KW. et al. (2003) The Journal of biological chemistry 278, 15905-10. PMID: 12609982 9. Kelsall, IR. et al. (2013) The EMBO journal 32, 2848-60. PMID: 24076655
Note	For research use only

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Datasheet

Arabidopsis thalian CUL2 Recombinant Protein (N-His).pdf

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Arabidopsis thalian CUL2 Recombinant Protein (N-His) (PR022012)

Overview

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References

Datasheet

SDS