Anti-ABCB6 Polyclonal Antibody (HV027014)

Name: Anti-ABCB6 Polyclonal Antibody
Brand: abinScience
SKU: HV027014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HV027014

Description

Anti-ABCB6 Polyclonal Antibody (HV027014) is a rabbit polyclonal antibody detecting ABCB6 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human ABCB6 (Ile590-Arg842).

Target

UMAT, ATP-binding cassette sub-family B member 6, mitochondrial, MTABC3, Mitochondrial ABC transporter 3, ABCB6, P-glycoprotein-related protein, Mt-ABC transporter 3, PRP, Ubiquitously-expressed mammalian ABC half transporter

Purification

Purified by antigen affinity column.

Accession

Q9NP58

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

ATP-binding cassette sub-family B member 6 (ABCB6) is a ~93 kDa protein. ATP-dependent transporter that catalyzes the transport of a broad-spectrum of porphyrins from the cytoplasm to the extracellular space through the plasma membrane or into the vesicle lumen. May also function as an ATP-dependent importer of porphyrins from the cytoplasm into the mitochondria, in turn may participate in the de novo heme biosynthesis regulation and in the coordination of heme and iron homeostasis during phenylhydrazine stress. May also play a key role in the early steps of melanogenesis producing PMEL amyloid fibrils. In vitro, it confers to cells a resistance to toxic metal such as arsenic and cadmium and against chemotherapeutics agent such as 5-fluorouracil, SN-38 and vincristin. In addition may play a role in the transition metal homeostasis.

1. Paterson, JK. et al. (2007) Biochemistry 46, 9443-52. PMID: 17661442

2. Chavan, H. et al. (2013) The Journal of biological chemistry 288, 22658-69. PMID: 23792964

3. Fukuda, Y. et al. (2016) Nature communications 7, 12353. PMID: 27507172

4. Wang, C. et al. (2020) Protein science : a publication of the Protein Society 29, 2363-2374. PMID: 33007128

5. Mitsuhashi, N. et al. (2000) The Journal of biological chemistry 275, 17536-40. PMID: 10837493

6. Krishnamurthy, PC. et al. (2006) Nature 443, 586-9. PMID: 17006453

7. Bergam, P. et al. (2018) Journal of molecular biology 430, 3802-3818. PMID: 29940187

8. Chavan, H. et al. (2011) Toxicological sciences : an official journal of the Society of Toxicology 120, 519-28. PMID: 21266531

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-ABCB6 polyclonal antibody at 1μg/ml on various samples.
Lane 1: Hela cell lysate
Lane 2: HepG2 cell lysate
Lane 3: HL60 cell lysate

References

Datasheet

Anti-ABCB6 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-ABCB6 Polyclonal Antibody (HV027014)

Overview

Images

References

Datasheet

SDS

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