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Anti-ABCB9 Polyclonal Antibody (HV453014)

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Overview
Catalog No.HV453014
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human ABCB9 (Val504-Ala766).
Target ATP-binding cassette transporter 9, ABC transporter 9 protein, ATP-binding cassette sub-family B member 9, hABCB9, TAP-like protein, ABCB9, KIAA1520, TAPL
Purification Purified by antigen affinity column.
Accession Q9NP78
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

ABC-type oligopeptide transporter ABCB9 is a ~84 kDa protein. ATP-dependent low-affinity peptide transporter which translocates a broad spectrum of peptides from the cytosol to the lysosomal lumen for degradation. Displays a broad peptide length specificity from 6-mer up to at least 59-mer peptides with an optimum of 23-mers. Binds and transports smaller and larger peptides with the same affinity. Favors positively charged, aromatic or hydrophobic residues in the N- and C-terminal positions whereas negatively charged residues as well as asparagine and methionine are not favored.

1. Wolters, JC. et al. (2005) The Journal of biological chemistry 280, 23631-6. PMID: 15863492
2. Demirel, O. et al. (2007) The Journal of biological chemistry 282, 37836-43. PMID: 17977821
3. Zhao, C. et al. (2008) The Journal of biological chemistry 283, 17083-91. PMID: 18434309
4. Demirel, Ö. et al. (2012) Journal of cell science 125, 4230-40. PMID: 22641697
5. Zollmann, T. et al. (2015) Proceedings of the National Academy of Sciences of the United States of America 112, 2046-51. PMID: 25646430
6. Bock, C. et al. (2018) Scientific reports 8, 15662. PMID: 30353140
7. Graab, P. et al. (2019) The Journal of biological chemistry 294, 7308-7323. PMID: 30877195
8. Bock, C. et al. (2019) Scientific reports 9, 11884. PMID: 31417173
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
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C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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