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Anti-ABCE1 Polyclonal Antibody (HX969014)

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Overview
Catalog No.HX969014
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human ABCE1 (Ile389-Asn556).
Target HuHP68, RNS4I, RNASEL1, RNase L inhibitor, 2'-5'-oligoadenylate-binding protein, RLI, ABCE1, ATP-binding cassette sub-family E member 1, RNASELI, Ribonuclease 4 inhibitor
Purification Purified by antigen affinity column.
Accession P61221
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

ATP-binding cassette sub-family E member 1 (ABCE1) is a ~67 kDa protein. Nucleoside-triphosphatase (NTPase) involved in ribosome recycling by mediating ribosome disassembly. Able to hydrolyze ATP, GTP, UTP and CTP. Splits ribosomes into free 60S subunits and tRNA- and mRNA-bound 40S subunits. Acts either after canonical termination facilitated by release factors (ETF1/eRF1) or after recognition of stalled and vacant ribosomes by mRNA surveillance factors (PELO/Pelota). Involved in the No-Go Decay (NGD) pathway: recruited to stalled ribosomes by the Pelota-HBS1L complex, and drives the disassembly of stalled ribosomes, followed by degradation of damaged mRNAs as part of the NGD pathway.

1. Pisarev, AV. et al. (2010) Molecular cell 37, 196-210. PMID: 20122402
2. Pisareva, VP. et al. (2011) The EMBO journal 30, 1804-17. PMID: 21448132
3. Wu, Z. et al. (2018) Cell metabolism 28, 130-144.e7. PMID: 29861391
5. Le Roy, F. et al. (2001) The Journal of biological chemistry 276, 48473-82. PMID: 11585831
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Volume
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g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-ABCE1 Polyclonal Antibody

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