Anti-ACAA2 Polyclonal Antibody (HW427014)

Name: Anti-ACAA2 Polyclonal Antibody
Brand: abinScience
SKU: HW427014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HW427014

Description

Anti-ACAA2 Polyclonal Antibody (HW427014) is a rabbit polyclonal antibody detecting ACAA2 in ELISA, IHC, WB. Suitable for Bovine, Human, Mouse, Pongo abelii, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human ACAA2 (Arg90-Ala397).

Target

Acetyl-CoA acyltransferase, Mitochondrial 3-oxoacyl-CoA thiolase, T1, Beta-ketothiolase, 3-ketoacyl-CoA thiolase, mitochondrial, Acyl-CoA hydrolase, mitochondrial, ACAA2, Acetyl-CoA acetyltransferase

Purification

Purified by antigen affinity column.

Accession

P42765

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

3-ketoacyl-CoA thiolase, mitochondrial (ACAA2) is a ~41 kDa protein. In the production of energy from fats, this is one of the enzymes that catalyzes the last step of the mitochondrial beta-oxidation pathway, an aerobic process breaking down fatty acids into acetyl-CoA (Probable). Using free coenzyme A/CoA, catalyzes the thiolytic cleavage of medium- to long-chain unbranched 3-oxoacyl-CoAs into acetyl-CoA and a fatty acyl-CoA shortened by two carbon atoms (Probable). Also catalyzes the condensation of two acetyl-CoA molecules into acetoacetyl-CoA and could be involved in the production of ketone bodies (Probable). Also displays hydrolase activity on various fatty acyl-CoAs. Thereby, could be responsible for the production of acetate in a side reaction to beta-oxidation (Probable).

1. Kiema, TR. et al. (2014) Acta crystallographica. Section D, Biological crystallography 70, 3212-25. PMID: 25478839

2. Cao, W. et al. (2008) Biochimica et biophysica acta 1780, 873-80. PMID: 18371312

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-ACAA2 polyclonal antibody at 1μg/ml on various samples.
Lane 1: Mouse heart lysate
Lane 2: Mouse liver lysate
Lane 3: Rat heart lysate
Lane 4: Rat liver lysate

References

Datasheet

Anti-ACAA2 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-ACAA2 Polyclonal Antibody (HW427014)

Overview

Images

References

Datasheet

SDS

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