Anti-ACAT1 Polyclonal Antibody (HB220014)

Name: Anti-ACAT1 Polyclonal Antibody
Brand: abinScience
SKU: HB220014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HB220014

Description

Anti-ACAT1 Polyclonal Antibody (HB220014) is a rabbit polyclonal antibody detecting ACAT1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Macaca fascicularis.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human ACAT1 (Ala111-Leu427).

Target

ACAT, Acetoacetyl-CoA thiolase, MAT, Acetyl-CoA acetyltransferase, mitochondrial, ACAT1, T2

Purification

Purified by antigen affinity column.

Accession

P24752

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Acetyl-CoA acetyltransferase, mitochondrial (ACAT1) is a ~45 kDa protein. This is one of the enzymes that catalyzes the last step of the mitochondrial beta-oxidation pathway, an aerobic process breaking down fatty acids into acetyl-CoA. Using free coenzyme A/CoA, catalyzes the thiolytic cleavage of medium- to long-chain 3-oxoacyl-CoAs into acetyl-CoA and a fatty acyl-CoA shortened by two carbon atoms. The activity of the enzyme is reversible and it can also catalyze the condensation of two acetyl-CoA molecules into acetoacetyl-CoA. Thereby, it plays a major role in ketone body metabolism.

1. Fukao, T. et al. (1991) Biochemical and biophysical research communications 179, 124-9. PMID: 1715688

2. Wakazono, A. et al. (1995) Human mutation 5, 34-42. PMID: 7728148

3. Fukao, T. et al. (1998) Human mutation 12, 245-54. PMID: 9744475

4. Haapalainen, AM. et al. (2007) Biochemistry 46, 4305-21. PMID: 17371050

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-ACAT1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Caco2 cell lysate
Lane 2: Mouse heart lysate
Lane 3: Mouse liver lysate
Lane 4: Rat heart lysate
Lane 5: Rat skeletal muscle lysate
Lane 6: Rat liver lysate

References

Datasheet

Anti-ACAT1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-ACAT1 Polyclonal Antibody (HB220014)

Overview

Images

References

Datasheet

SDS

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