Anti-ACO1 Polyclonal Antibody (HB808014)

Name: Anti-ACO1 Polyclonal Antibody
Brand: abinScience
SKU: HB808014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HB808014

Description

Anti-ACO1 Polyclonal Antibody (HB808014) is a rabbit polyclonal antibody detecting ACO1 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant human ACO1 (Lys26-Ile243).

Target

Cytoplasmic aconitate hydratase, Aconitase, EC:4.2.1.3, Citrate hydro-lyase, Ferritin repressor protein, Iron regulatory protein 1, IRP1, Iron-responsive element-binding protein 1, IRE-BP 1, ACO1, IREB1

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

P21399, P28271, Q63270

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Cytoplasmic aconitate hydratase (ACO1) is a ~98 kDa protein. Bifunctional iron sensor that switches between 2 activities depending on iron availability. Iron deprivation, promotes its mRNA binding activity through which it regulates the expression of genes involved in iron uptake, sequestration and utilization. Binds to iron-responsive elements (IRES) in the untranslated region of target mRNAs preventing for instance the translation of ferritin and aminolevulinic acid synthase and stabilizing the transferrin receptor mRNA.

1. Haile, DJ. et al. (1992) Proceedings of the National Academy of Sciences of the United States of America 89, 11735-9. PMID: 1281544

2. Kaptain, S. et al. (1991) Proceedings of the National Academy of Sciences of the United States of America 88, 10109-13. PMID: 1946430

3. Philpott, CC. et al. (1994) Proceedings of the National Academy of Sciences of the United States of America 91, 7321-5. PMID: 8041788

4. Stehling, O. et al. (2013) Cell metabolism 18, 187-98. PMID: 23891004

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-ACO1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Hela cell lysate
Lane 2: U2OS cell lysate
Lane 3: 293T cell lysate
Lane 4: HepG2 cell lysate
Lane 5: A549 cell lysate
Lane 6: Mouse liver lysate
Lane 7: Rat liver lysate

References

Datasheet

Anti-ACO1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-ACO1 Polyclonal Antibody (HB808014)

Overview

Images

References

Datasheet

SDS