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Anti-ACOX1 Polyclonal Antibody (HS771014)

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Overview
Catalog No.HS771014
Description
Anti-ACOX1 Polyclonal Antibody (HS771014) is a rabbit polyclonal antibody detecting ACOX1 in ELISA, IHC, WB. Suitable for Bovine, Cavia porcellus, Human, Pongo abelii, and Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human ACOX1 (Tyr275-Leu660).
Target ACOX1, Palmitoyl-CoA oxidase, SCOX, AOX, ACOX, Peroxisomal acyl-coenzyme A oxidase 1, Straight-chain acyl-CoA oxidase
Purification Purified by antigen affinity column.
Accession Q15067
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Peroxisomal acyl-coenzyme A oxidase 1 (ACOX1) is a ~74 kDa protein. Involved in the initial and rate-limiting step of peroxisomal beta-oxidation of straight-chain saturated and unsaturated very-long-chain fatty acids. Catalyzes the desaturation of fatty acyl-CoAs that have a saturated bond between C2 and C3 (2,3-saturated acyl-CoA) to 2-trans-enoyl-CoAs ((2E)-enoyl-CoAs), and donates electrons directly to molecular oxygen (O(2)), thereby producing hydrogen peroxide (H(2)O(2)).

1. Ferdinandusse, S. et al. (2004) Journal of lipid research 45, 1104-11. PMID: 15060085
2. Ferdinandusse, S. et al. (2007) Human mutation 28, 904-12. PMID: 17458872
3. Oaxaca-Castillo, D. et al. (2007) Biochemical and biophysical research communications 360, 314-9. PMID: 17603022
4. Chung, HL. et al. (2020) Neuron 106, 589-606.e6. PMID: 32169171
5. Morita, A. et al. (2021) Brain & development 43, 475-481. PMID: 33234382
6. Chu, R. et al. (1995) The Journal of biological chemistry 270, 4908-15. PMID: 7876265
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-ACOX1 Polyclonal Antibody

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