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Anti-ACSL4 Polyclonal Antibody (HT115014)

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Overview
Catalog No.HT115014
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human ACSL4 (Lys500-Lys711).
Target ACS4, ACSL4, Long-chain-fatty-acid--CoA ligase 4, LACS 4, Arachidonate--CoA ligase, FACL4, Long-chain acyl-CoA synthetase 4, LACS4
Purification Purified by antigen affinity column.
Accession O60488
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Long-chain-fatty-acid--CoA ligase 4 (ACSL4) is a ~79 kDa protein. Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoA for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially activates arachidonate and eicosapentaenoate as substrates. Preferentially activates 8,9-EET > 14,15-EET > 5,6-EET > 11,12-EET. Modulates glucose-stimulated insulin secretion by regulating the levels of unesterified EETs. Modulates prostaglandin E2 secretion.

1. Golej, DL. et al. (2011) Journal of lipid research 52, 782-93. PMID: 21242590
2. Nakahara, K. et al. (2012) Molecular cell 46, 461-71. PMID: 22633490
3. Ohkuni, A. et al. (2013) Biochemical and biophysical research communications 442, 195-201. PMID: 24269233
4. Shimbara-Matsubayashi, S. et al. (2019) Biological & pharmaceutical bulletin 42, 850-855. PMID: 31061331
5. Li, Z. et al. (2024) Nature chemical biology 20, 1341-1352. PMID: 38720107
6. Doll, S. et al. (2017) Nature chemical biology 13, 91-98. PMID: 27842070
7. Zhang, HL. et al. (2022) Nature cell biology 24, 88-98. PMID: 35027735
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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V₁ (Stock Vol.)
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