

| Catalog No. | HT115014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Species reactivity | Human, Mouse, Rat | ||||||||
| Applications | ELISA, IHC, WB | ||||||||
| Host species | Rabbit | ||||||||
| Isotype | IgG | ||||||||
| Clonality | Polyclonal | ||||||||
| Immunogen | E. coli - derived recombinant Human ACSL4 (Lys500-Lys711). | ||||||||
| Target | ACS4, ACSL4, Long-chain-fatty-acid--CoA ligase 4, LACS 4, Arachidonate--CoA ligase, FACL4, Long-chain acyl-CoA synthetase 4, LACS4 | ||||||||
| Purification | Purified by antigen affinity column. | ||||||||
| Accession | O60488 | ||||||||
| Form | Liquid | ||||||||
| Storage buffer | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
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| Product Usage Information |
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| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| Background | Long-chain-fatty-acid--CoA ligase 4 (ACSL4) is a ~79 kDa protein. Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoA for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially activates arachidonate and eicosapentaenoate as substrates. Preferentially activates 8,9-EET > 14,15-EET > 5,6-EET > 11,12-EET. Modulates glucose-stimulated insulin secretion by regulating the levels of unesterified EETs. Modulates prostaglandin E2 secretion. 1. Golej, DL. et al. (2011) Journal of lipid research 52, 782-93. PMID: 21242590 2. Nakahara, K. et al. (2012) Molecular cell 46, 461-71. PMID: 22633490 3. Ohkuni, A. et al. (2013) Biochemical and biophysical research communications 442, 195-201. PMID: 24269233 4. Shimbara-Matsubayashi, S. et al. (2019) Biological & pharmaceutical bulletin 42, 850-855. PMID: 31061331 5. Li, Z. et al. (2024) Nature chemical biology 20, 1341-1352. PMID: 38720107 6. Doll, S. et al. (2017) Nature chemical biology 13, 91-98. PMID: 27842070 7. Zhang, HL. et al. (2022) Nature cell biology 24, 88-98. PMID: 35027735 | ||||||||
| Note | For research use only. |
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