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Anti-ACSS1 Polyclonal Antibody (HV651014)

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Overview
Catalog No.HV651014
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human ACSS1 (Phe517-Lys689).
Target Acetyl-CoA synthetase 2, ACSS1, ACAS2L, Acetate--CoA ligase 2, Propionate--CoA ligase, KIAA1846, AceCS2, Acetyl-coenzyme A synthetase 2-like, mitochondrial, Acyl-CoA synthetase short-chain family member 1
Purification Purified by antigen affinity column.
Accession Q9NUB1
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Acetyl-coenzyme A synthetase 2-like, mitochondrial (ACSS1) is a ~74 kDa protein. Catalyzes the synthesis of acetyl-CoA from short-chain fatty acids. Acetate is the preferred substrate. Can also utilize propionate with a much lower affinity. Provides acetyl-CoA that is utilized mainly for oxidation under ketogenic conditions. Involved in thermogenesis under ketogenic conditions, using acetate as a vital fuel when carbohydrate availability is insufficient.

1. Schwer, B. et al. (2006) Proceedings of the National Academy of Sciences of the United States of America 103, 10224-10229. PMID: 16788062
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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