Anti-ACTG1 Polyclonal Antibody (HX058014) is a rabbit polyclonal antibody detecting ACTG1 in ELISA, IHC, WB. Suitable for Human, Mouse, Dog, Rat, and Absidia glauca.
Highlights
●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.
Species reactivity
Human, Mouse, Rat
Applications
ELISA, IHC, WB
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human ACTG1 (Met1-Phe375).
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application
Dilution
ELISA
1:5000-1:20000
IHC
1:50-1:500
WB
1:500-1:2000
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background
Actin, cytoplasmic 1 (ACTG1) is a ~41 kDa protein. Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells. Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction. In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA. Plays a role in the assembly of the gamma-tubulin ring complex (gTuRC), which regulates the minus-end nucleation of alpha-beta tubulin heterodimers that grow into microtubule protafilaments. Part of the ACTR1A/ACTB filament around which the dynactin complex is built.
1. Baarlink, C. et al. (2013) Science (New York, N.Y.) 340, 864-7. PMID: 23558171
2. Plessner, M. et al. (2015) The Journal of biological chemistry 290, 11209-16. PMID: 25759381
Note
For research use only.
Images
Western blot
Western blot analysis was performed using anti-ACTG1 polyclonal antibody at 1μg/ml on various samples. Lane 1: recombinant human ACTG1 (Catalog No: HX058012) Lane 2: PC12 cell lysate Lane 3: MCF7 cell lysate
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