Anti-ADPRS Polyclonal Antibody (HB443014)

Name: Anti-ADPRS Polyclonal Antibody
Brand: abinScience
SKU: HB443014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HB443014

Description

Anti-ADPRS Polyclonal Antibody (HB443014) is a rabbit polyclonal antibody detecting ADP-ribosylhydrolase ARH3; ADP-ribose glycohydrolase ARH3; ADP-ribosylhydrolase 3; O-acetyl-ADP-ribose deacetylase ARH3; 3.5.1.-; Poly(ADP-ribose) glycohydrolase ARH3; 3.2.1.143; [Protein ADP-ribosylarginine] hydrolase-like protein 2; [Protein ADP-ribosylserine] hydrolase; 3.2.2.-; ADPRS; ADPRHL2; ARH3 in ELISA, IHC, WB. Suitable for Human, Bovine, and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant human ADPRS (Ser19-Ser363).

Target

ADP-ribosylhydrolase ARH3; ADP-ribose glycohydrolase ARH3; ADP-ribosylhydrolase 3; O-acetyl-ADP-ribose deacetylase ARH3; 3.5.1.-; Poly(ADP-ribose) glycohydrolase ARH3; 3.2.1.143; [Protein ADP-ribosylarginine] hydrolase-like protein 2; [Protein ADP-ribosylserine] hydrolase; 3.2.2.-; ADPRS; ADPRHL2; ARH3

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q9NX46, Q3SYV9, Q8CG72

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

ADP-ribosylhydrolase ARH3 (ADPRS) is a ~38 kDa protein. ADP-ribosylhydrolase that preferentially hydrolyzes the scissile alpha-O-linkage attached to the anomeric C1'' position of ADP-ribose and acts on different substrates, such as proteins ADP-ribosylated on serine and threonine, free poly(ADP-ribose) and O-acetyl-ADP-D-ribose. Specifically acts as a serine mono-ADP-ribosylhydrolase by mediating the removal of mono-ADP-ribose attached to serine residues on proteins, thereby playing a key role in DNA damage response. Serine ADP-ribosylation of proteins constitutes the primary form of ADP-ribosylation of proteins in response to DNA damage. Does not hydrolyze ADP-ribosyl-arginine, -cysteine, -diphthamide, or -asparagine bonds. Also able to degrade protein free poly(ADP-ribose), which is synthesized in response to DNA damage: free poly(ADP-ribose) acts as a potent cell death signal and its degradation by ADPRHL2 protects cells from poly(ADP-ribose)-dependent cell death, a process named parthanatos.

1. Kasamatsu, A. et al. (2011) The Journal of biological chemistry 286, 21110-7. PMID: 21498885

2. Pourfarjam, Y. et al. (2018) The Journal of biological chemistry 293, 12350-12359. PMID: 29907568

3. Wang, M. et al. (2018) The Journal of biological chemistry 293, 14470-14480. PMID: 30045870

4. Danhauser, K. et al. (2018) American journal of human genetics 103, 817-825. PMID: 30401461

5. Mashimo, M. et al. (2019) JCI insight 4. PMID: 30830864

6. Bonfiglio, JJ. et al. (2020) Cell 183, 1086-1102.e23. PMID: 33186521

7. Voorneveld, J. et al. (2021) Chemistry (Weinheim an der Bergstrasse, Germany) 27, 10621-10627. PMID: 33769608

8. Pourfarjam, Y. et al. (2021) The Journal of biological chemistry 296, 100692. PMID: 33894202

9. Prokhorova, E. et al. (2021) Molecular cell 81, 2640-2655.e8. PMID: 34019811

10. Rack, JGM. et al. (2021) Nature communications 12, 4581. PMID: 34321462

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-ADPRS polyclonal antibody at 1ug/mL on various samples.
Lane 1: Rat brain lysate
Lane 2: Rat heart lysate

References

Datasheet

Anti-ADPRS Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-ADPRS Polyclonal Antibody (HB443014)

Overview

Images

References

Datasheet

SDS

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