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Anti-Mouse beta-2 Adrenergic R/ADRB2 Polyclonal Antibody (MY357014)

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Overview
Catalog No.MY357014
Description
Anti-ADRB2 Polyclonal Antibody (MY357014) is a rabbit polyclonal antibody detecting Beta-2 adrenergic receptor; Beta-2 adrenoreceptor; Beta-2 adrenoceptor; Adrb2; Adrb2r in ELISA, IHC, WB. Suitable for Rat and Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityMouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant mouse ADRB2 (Pro330-Leu418).
Target Beta-2 adrenergic receptor; Beta-2 adrenoreceptor; Beta-2 adrenoceptor; Adrb2; Adrb2r
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Purified by antigen affinity column.
Accession P18762, P10608
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Beta-2 adrenergic receptor (ADRB2) is a ~46 kDa protein. G protein-coupled receptor for catecholamines that couples to both G(s) and G(i) proteins, activating bifurcated signaling pathways. ADRB2 binds epinephrine (Epi) with an approximately 30-fold greater affinity than norepinephrine (NE). In the heart, Epi- and NE-activated ADRB2 induces rapid and slow cardiomyocyte contraction rate, respectively. Both NE and Epi promote coupling to G(s)/PKA pathway to regulate myocyte contraction rate. Epi also promotes ADRB2 coupling to G(i) proteins to exert cardioprotective effects especially in the conditions of hypoxia and oxidative stress through the G(i)/PI3K/Akt signaling pathway.

1. Zhu, WZ. et al. (2001) Proceedings of the National Academy of Sciences of the United States of America 98, 1607-12. PMID: 11171998
2. Wang, Y. et al. (2008) The Journal of biological chemistry 283, 1799-807. PMID: 18056263
3. Allen, JM. et al. (1988) The EMBO journal 7, 133-8. PMID: 2834198
Note For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Mouse beta-2 Adrenergic R/ADRB2 Polyclonal Antibody

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