Anti-AFG2A Polyclonal Antibody (HA744014)

Name: Anti-AFG2A Polyclonal Antibody
Brand: abinScience
SKU: HA744014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HA744014

Description

Anti-AFG2A Polyclonal Antibody (HA744014) is a rabbit polyclonal antibody detecting ATPase family gene 2 protein homolog A; 3.6.4.10; AFG2 AAA ATPase homolog A; Ribosome biogenesis protein SPATA5; Spermatogenesis-associated factor protein; Spermatogenesis-associated protein 5; AFG2A; SPAF; SPATA5 in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant human AFG2A (Thr351-Leu893).

Target

ATPase family gene 2 protein homolog A; 3.6.4.10; AFG2 AAA ATPase homolog A; Ribosome biogenesis protein SPATA5; Spermatogenesis-associated factor protein; Spermatogenesis-associated protein 5; AFG2A; SPAF; SPATA5

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q8NB90, Q3UMC0

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

ATPase family gene 2 protein homolog A (AFG2A) is a ~97 kDa protein. ATP-dependent chaperone part of the 55LCC heterohexameric ATPase complex which is chromatin-associated and promotes replisome proteostasis to maintain replication fork progression and genome stability. Required for replication fork progression, sister chromatid cohesion, and chromosome stability. The ATPase activity is specifically enhanced by replication fork DNA and is coupled to cysteine protease-dependent cleavage of replisome substrates in response to replication fork damage. Uses ATPase activity to process replisome substrates in S-phase, facilitating their proteolytic turnover from chromatin to ensure DNA replication and mitotic fidelity. Plays an essential role in the cytoplasmic maturation steps of pre-60S ribosomal particles by promoting the release of shuttling protein RSL24D1/RLP24 from the pre-ribosomal particles.

1. Krishnamoorthy, V. et al. (2024) Cell 187, 2250-2268.e31. PMID: 38554706

2. Ni, C. et al. (2022) Cell reports 38, 110597. PMID: 35354024

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-AFG2A polyclonal antibody at 1ug/mL on various samples.
Lane 1: 293T cell lysate
Lane 2: LO2 cell lysate
Lane 3: Mouse liver lysate
Lane 4: Mouse brain lysate

References

Datasheet

Anti-AFG2A Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Assay Kits

Anti-AFG2A Polyclonal Antibody (HA744014)

Overview

Images

References

Datasheet

SDS

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