Anti-AGK Polyclonal Antibody (HS733014)

Name: Anti-AGK Polyclonal Antibody
Brand: abinScience
SKU: HS733014
Price: 168 USD
Availability: InStock

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Datasheet
SDS

Overview

Catalog No.

HS733014

Description

Anti-AGK Polyclonal Antibody (HS733014) is a rabbit polyclonal antibody detecting AGK in ELISA, IHC, WB. Suitable for Human, Mouse, and Pongo abelii.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human AGK (Thr15-Pro300).

Target

MULK, Acylglycerol kinase, mitochondrial, hAGK, Multiple substrate lipid kinase, HsMuLK, MuLK, Multi-substrate lipid kinase, AGK

Purification

Purified by antigen affinity column.

Accession

Q53H12

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Acylglycerol kinase, mitochondrial (AGK) is a ~47 kDa protein. Lipid kinase that can phosphorylate both monoacylglycerol and diacylglycerol to form lysophosphatidic acid (LPA) and phosphatidic acid (PA), respectively. Does not phosphorylate sphingosine. Phosphorylates ceramide. Phosphorylates 1,2-dioleoylglycerol more rapidly than 2,3-dioleoylglycerol. Independently of its lipid kinase activity, acts as a component of the TIM22 complex.

1. Bektas, M. et al. (2005) The Journal of cell biology 169, 801-11. PMID: 15939762

2. Vukotic, M. et al. (2017) Molecular cell 67, 471-483.e7. PMID: 28712724

3. Kang, Y. et al. (2017) Molecular cell 67, 457-470.e5. PMID: 28712726

Note

For research use only.

Images

References

Datasheet

Anti-AGK Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-AGK Polyclonal Antibody (HS733014)

Overview

Images

References

Datasheet

SDS

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