Anti-AIF1/IBA1 Polyclonal Antibody (HX192014)

Name: Anti-AIF1/IBA1 Polyclonal Antibody
Brand: abinScience
SKU: HX192014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HX192014

Description

Anti-AIF1/IBA1 Polyclonal Antibody (HX192014) is a rabbit polyclonal antibody detecting AIF1 in ELISA, IHC, WB, IF. Suitable for Mouse, Bovine, Human, Macaca mulatta, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human

Applications

ELISA, IHC, WB, IF

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human AIF1/IBA1 (Met1-Pro147).

Target

Ionized calcium-binding adapter molecule 1, AIF1, AIF-1, Allograft inflammatory factor 1, IBA1, G1, Protein G1

Purification

Purified by antigen affinity column.

Accession

P55008

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Allograft inflammatory factor 1 (AIF1/IBA1) is a ~16 kDa protein. Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function.

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-AIF1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: THP-1 cell lysate

References

Datasheet

Anti-AIF1/IBA1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-AIF1/IBA1 Polyclonal Antibody (HX192014)

Overview

Images

References

Datasheet

SDS

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