Anti-AIF1 Polyclonal Antibody (MX192014)

Name: Anti-AIF1 Polyclonal Antibody
Brand: abinScience
SKU: MX192014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

MX192014

Description

Anti-AIF1 Polyclonal Antibody (MX192014) is a rabbit polyclonal antibody detecting Allograft inflammatory factor 1; AIF-1; Ionized calcium-binding adapter molecule 1; Aif1; Iba1 in ELISA, IHC, WB. Suitable for Human, Rat, Pig, and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant mouse AIF1 (Ser2-Pro147).

Target

Allograft inflammatory factor 1; AIF-1; Ionized calcium-binding adapter molecule 1; Aif1; Iba1

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

P55009, P81076, O70200, P55008

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Allograft inflammatory factor 1 (AIF1) is a ~16 kDa protein. Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play an role in macrophage activation and function.

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-AIF1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Mouse spleen lysate
Lane 2: Rat spleen lysate

References

Datasheet

Anti-AIF1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-AIF1 Polyclonal Antibody (MX192014)

Overview

Images

References

Datasheet

SDS

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