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Anti-AK3 Polyclonal Antibody (HV747014)

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Anti-AK3 Polyclonal Antibody
Anti-AK3 Polyclonal Antibody
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Overview
Catalog No.HV747014
Description
Anti-AK3 Polyclonal Antibody (HV747014) is a rabbit polyclonal antibody detecting AK3 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Pongo abelii.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human AK3 (Leu8-Pro227).
Target GTP:AMP phosphotransferase AK3, mitochondrial, AK 3, AK6, Adenylate kinase 3 alpha-like 1, AK3, AKL3L, AK3L1, Adenylate kinase 3
Purification Purified by antigen affinity column.
Accession Q9UIJ7
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

GTP:AMP phosphotransferase AK3, mitochondrial is a ~25 kDa protein. Mitochondrial adenylate kinase with a specific GTP:AMP phosphotransferase activity. Could also use ITP as phosphate donor. Its physiological function is to recycle GTP into GDP which is necessary for the TCA cycle in the mitochondrial matrix (Probable).

1. Noma, T. et al. (2001) The Biochemical journal 358, 225-32. PMID: 11485571
2. Rogne, P. et al. (2020) Biochemistry 59, 3570-3581. PMID: 32822537
Note For research use only.
Images
  • Anti-AK3 Polyclonal Antibody

    Western blot

    Lane 1: HepG2 cell lysate
    Lane 2: A549 cell lysate
    Lane 3: BxPC-3 cell lysate
    Lane 4: Mouse heart lysate
    Lane 5: Mouse liver lysate
    Lane 6: Mouse kidney lysate
    Lane 7: Rat heart lysate
    Lane 8: Rat liver lysate

References
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-AK3 Polyclonal Antibody

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