Anti-AKR1C4 Polyclonal Antibody (HB616014)

Name: Anti-AKR1C4 Polyclonal Antibody
Brand: abinScience
SKU: HB616014
Price: 168 USD
Availability: InStock

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SDS

Overview

Catalog No.

HB616014

Description

Anti-AKR1C4 Polyclonal Antibody (HB616014) is a rabbit polyclonal antibody detecting AKR1C4 in ELISA, IHC, WB. Suitable for Human and Macaca fascicularis.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human AKR1C4 (Met1-Tyr323).

Target

3-alpha-HSD1, 3alpha-hydroxysteroid 3-dehydrogenase, DD4, Aldo-keto reductase family 1 member C4, AKR1C4, DD-4, CDR, HAKRA, Chlordecone reductase, 3-alpha-hydroxysteroid dehydrogenase type I, CHDR, Dihydrodiol dehydrogenase 4

Purification

Purified by antigen affinity column.

Accession

P17516

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Aldo-keto reductase family 1 member C4 (AKR1C4) is a ~37 kDa protein. Cytosolic aldo-keto reductase that catalyzes NADPH-dependent reduction of ketosteroids to hydroxysteroids. Displays broad substrate specificity with distinct positional and stereochemistry, primarily generating 3alpha/beta-, 17beta- and 20alpha-hydroxysteroids. Required for male sex determination as a component of the 'backdoor' androgen biosynthesis pathway that generates 5alpha-dihydrotestosterone (5alpha-DHT) via pregnanes. Acts together with AKR1C2 to convert 5alpha-dihydroprogesterone (5alpha-DHP) to 3alpha-hydroxy-5alpha-pregnan-20-one (3alpha,5alpha-THP/allopregnanolone), leading to 5alpha-DHT secretion necessary for embryonic gonad differentiation into testis. May regulate the concentrations of circulating neurosteroids.

1. Kume, T. et al. (1999) Pharmacogenetics 9, 763-71. PMID: 10634139

2. Penning, TM. et al. (2000) The Biochemical journal 351, 67-77. PMID: 10998348

3. Dufort, I. et al. (2001) The Journal of clinical endocrinology and metabolism 86, 841-6. PMID: 11158055

4. Steckelbroeck, S. et al. (2004) The Journal of biological chemistry 279, 10784-95. PMID: 14672942

5. Binstock, JM. et al. (1992) Biochemical and biophysical research communications 187, 760-6. PMID: 1530633

6. Higaki, Y. et al. (2003) Chemico-biological interactions 143-144, 503-13. PMID: 12604236

7. Jin, Y. et al. (2009) The Journal of biological chemistry 284, 10013-22. PMID: 19218247

8. Flück, CE. et al. (2011) American journal of human genetics 89, 201-18. PMID: 21802064

9. Khanna, M. et al. (1995) The Journal of biological chemistry 270, 20162-8. PMID: 7650035

10. Endo, S. et al. (2020) The Journal of steroid biochemistry and molecular biology 199, 105586. PMID: 31926269

Note

For research use only.

Images

References

Datasheet

Anti-AKR1C4 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-AKR1C4 Polyclonal Antibody (HB616014)

Overview

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References

Datasheet

SDS

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