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Anti-ALDH3B1 Polyclonal Antibody (HW419014)

Anti-ALDH3B1 Polyclonal Antibody
Anti-ALDH3B1 Polyclonal Antibody
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Overview
Catalog No.HW419014
Description
Anti-ALDH3B1 Polyclonal Antibody (HW419014) is a rabbit polyclonal antibody detecting ALDH3B1 in WB, IHC, ELISA. Suitable for Human, Mouse, Rat, Dog, and Cat.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
  • Multi-Species — Cross-reactive for translational research.
Species reactivityHuman
ApplicationsWB,IHC,ELISA
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant ALDH3B1 (Met1-Glu459).
Target Aldehyde dehydrogenase family 3 member B1, EC:1.2.1.28, EC:1.2.1.5, EC:1.2.1.7, Aldehyde dehydrogenase 7, Long-chain fatty aldehyde dehydrogenase, EC:1.2.1.48, Medium-chain fatty aldehyde dehydrogenase, ALDH3B1
Endotoxin level Please contact with the lab for this information.
Purity >90% as determined by SDS-PAGE.
Purification Purified by antigen affinity column.
Accession P43353, Q1JPA0, Q5XI42
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Aldehyde dehydrogenase family 3 member B1 (ALDH3B1) is a ~51 kDa protein. Oxidizes medium and long chain saturated and unsaturated fatty aldehydes generated in the plasma membrane into non-toxic fatty acids. May have a protective role against the cytotoxicity induced by lipid peroxidation. Short-chain fatty aldehydes are not good substrates. Can use both NADP(+) and NAD(+) as electron acceptor in vitro, however in vivo preference will depend on their tissue levels. Low activity towards acetaldehyde and 3,4-dihydroxyphenylacetaldehyde.

1. Marchitti, SA. et al. (2007) Biochemical and biophysical research communications 356, 792-8. PMID: 17382292
2. Kitamura, T. et al. (2013) Biochimica et biophysica acta 1831, 1395-401. PMID: 23721920
Note For research use only.
Images
  • Anti-ALDH3B1 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-ALDH3B1 polyclonal antibody at 1ug/mL on recombinant ALDH3B1 (Catalog No. HW419012).

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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