Anti-ALDOC Polyclonal Antibody (HY519014)

Name: Anti-ALDOC Polyclonal Antibody
Brand: abinScience
SKU: HY519014
Price: 96 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HY519014

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human ALDOC (Asp34-Ser281).

Target

Brain-type aldolase, ALDOC, ALDC, Fructose-bisphosphate aldolase C

Purification

Purified by antigen affinity column.

Accession

P09972

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Fructose-bisphosphate aldolase C (ALDOC) is a ~39 kDa protein. Catalyzes the reversible conversion of beta-D-fructose 1,6-bisphosphate (FBP) into two triose phosphate and plays a key role in glycolysis and gluconeogenesis.

Note

For research use only.

Images

Immunofluorescence

Hela cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting ALDOC (HY519014) at a concentration of 24 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of ALDOC was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).
Western blot

Western blot analysis was performed using anti-ALDOC polyclonal antibody at 1μg/ml on various samples.
Lane 1: MCF7 cell lysate
Lane 2: LO2 cell lysate
Lane 3: k562 cell lysate
Lane 4: Jurkat cell lysate
Lane 5: Mouse brain lysate
Lane 6: Mouse kidney lysate
Lane 7: Mouse muscle lysate
Lane 8: Mouse heart lysate

References

Datasheet

Anti-ALDOC Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-ALDOC Polyclonal Antibody (HY519014)

Overview

Images

References

Datasheet

SDS

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