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Anti-AMBP Polyclonal Antibody (HY281014)

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Overview
Catalog No.HY281014
Description
Anti-AMBP Polyclonal Antibody (HY281014) is a rabbit polyclonal antibody detecting AMBP in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human AMBP (Val22-Val203).
Target AMBP, HCP, Complex-forming glycoprotein heterogeneous in charge, ITIL, Bikunin, Protein HC, EDC1, HI-30, Uronic-acid-rich protein, Protein AMBP, ITI-LC, Alpha-1 microglycoprotein
Purification Purified by antigen affinity column.
Accession P02760
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Protein AMBP (α-MG) is a ~38 kDa protein. Antioxidant and tissue repair protein with reductase, heme-binding and radical-scavenging activities. Removes and protects against harmful oxidants and repairs macromolecules in intravascular and extravascular spaces and in intracellular compartments. Intravascularly, plays a regulatory role in red cell homeostasis by preventing heme- and reactive oxygen species-induced cell damage. Binds and degrades free heme to protect fetal and adult red blood cells from hemolysis. Reduces extracellular methemoglobin, a Fe3+ (ferric) form of hemoglobin that cannot bind oxygen, back to the Fe2+ (ferrous) form deoxyhemoglobin, which has oxygen-carrying potential.

1. Allhorn, M. et al. (2002) Blood 99, 1894-901. PMID: 11877257
2. Allhorn, M. et al. (2005) Free radical biology & medicine 38, 557-67. PMID: 15683711
3. Olsson, MG. et al. (2011) PloS one 6, e27505. PMID: 22096585
4. Olsson, MG. et al. (2013) Antioxidants & redox signaling 18, 2017-28. PMID: 23157686
5. Rutardottir, S. et al. (2013) Free radical research 47, 541-50. PMID: 23642167
6. Cederlund, M. et al. (2015) Frontiers in physiology 6, 11. PMID: 25698971
7. Kristiansson, A. et al. (2021) Free radical biology & medicine 162, 149-159. PMID: 32092412
8. Kristiansson, A. et al. (2020) International journal of molecular sciences 21. PMID: 32823731
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-AMBP Polyclonal Antibody

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