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Anti-AOAH Polyclonal Antibody (HB957014)

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Anti-AOAH Polyclonal Antibody
Anti-AOAH Polyclonal Antibody
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Overview
Catalog No.HB957014
Description
Anti-AOAH Polyclonal Antibody (HB957014) is a rabbit polyclonal antibody detecting AOAH in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human AOAH (Ser24-Gln300).
Target AOAH, Acyloxyacyl hydrolase
Purification Purified by antigen affinity column.
Accession P28039
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Acyloxyacyl hydrolase (AOAH) is a ~65 kDa protein. Removes the secondary (acyloxyacyl-linked) fatty acyl chains from the lipid A region of bacterial lipopolysaccharides. By breaking down LPS, terminates the host response to bacterial infection and prevents prolonged and damaging inflammatory responses. In peritoneal macrophages, seems to be important for recovery from a state of immune tolerance following infection by Gram-negative bacteria.

1. Hagen, FS. et al. (1991) Biochemistry 30, 8415-23. PMID: 1883828
2. Gorelik, A. et al. (2018) Proceedings of the National Academy of Sciences of the United States of America 115, E896-E905. PMID: 29343645
3. Staab, JF. et al. (1994) The Journal of biological chemistry 269, 23736-42. PMID: 8089145
Note For research use only.
Images
  • Anti-AOAH Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-AOAH polyclonal antibody at 1ug/mL on various samples.
    Lane 1: HL-60 cell lysate
    Lane 2: THP-1 cell lysate
    Lane 3: A549 cell lysate
    Lane 4: HUVEC cell lysate
    Lane 5: BxPC-3 cell lysate

References
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-AOAH Polyclonal Antibody

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