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Anti-AP1S2 Polyclonal Antibody (HX026014)

Anti-AP1S2 Polyclonal Antibody
Anti-AP1S2 Polyclonal Antibody
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Overview
Catalog No.HX026014
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human AP1S2 (Met1-Thr157).
Target Sigma1B-adaptin, Sigma 1B subunit of AP-1 clathrin, AP1S2, Adaptor-related protein complex 1 subunit sigma-1B, Sigma-adaptin 1B, AP-1 complex subunit sigma-2, Clathrin assembly protein complex 1 sigma-1B small chain, Adaptor protein complex AP-1 subunit sigma-1B, Golgi adaptor HA1/AP1 adaptin sigma-1B subunit
Purification Purified by antigen affinity column.
Accession P56377
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

AP-1 complex subunit sigma-2 (AP1S2) is a ~18 kDa protein. Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.

Note For research use only.
Images
  • Anti-AP1S2 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-AP1S2 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Mouse lung lysate

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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