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Anti-AP2S1 Polyclonal Antibody (HW605014)

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Overview
Catalog No.HW605014
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human AP2S1 (Met1-Glu142).
Target Adaptor-related protein complex 2 subunit sigma, Clathrin assembly protein 2 sigma small chain, HA2 17 kDa subunit, AP2S1, Clathrin coat-associated protein AP17, Clathrin coat assembly protein AP17, AP-2 complex subunit sigma, Adaptor protein complex AP-2 subunit sigma, CLAPS2, AP17, Sigma2-adaptin, Plasma membrane adaptor AP-2 17 kDa protein
Purification Purified by antigen affinity column.
Accession P53680
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

AP-2 complex subunit sigma (AP2S1) is a ~17 kDa protein. Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components.

Note For research use only.
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Formula
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Anti-AP2S1 Polyclonal Antibody

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