Anti-APLN Polyclonal Antibody (HV404014)

Name: Anti-APLN Polyclonal Antibody
Brand: abinScience
SKU: HV404014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HV404014

Description

Anti-APLN Polyclonal Antibody (HV404014) is a rabbit polyclonal antibody detecting APLN in ELISA, IHC, WB. Suitable for Human, Bovine, Rattus norvegicus, and Mus musculus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human APLN (Ser24-Phe77).

Target

APEL, Apelin, APJ endogenous ligand, APLN

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q9ULZ1, Q9TUI9, Q9R0R3, Q9R0R4

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Apelin (APLN) is a ~8 kDa protein. Peptide hormone that functions as endogenous ligand for the G-protein-coupled apelin receptor (APLNR/APJ), that plays a role in cadiovascular homeostasis. Functions as a balanced agonist activating both G(i) protein pathway and beta-arrestin pathway of APLNR. Downstream G proteins activation, apelin can inhibit cAMP production and activate key intracellular effectors such as ERKs. On the other hand, APLNR activation induces beta-arrestin recruitment to the membrane leading to desensitization and internalization of the receptor. Apelin blunts cardiac hypertrophic induction from APLNR on response to pathological stimuli, but also induces myocardial hypertrophy under normal conditions.

1. Habata, Y. et al. (1999) Biochimica et biophysica acta 1452, 25-35. PMID: 10525157

2. Scimia, MC. et al. (2012) Nature 488, 394-8. PMID: 22810587

3. Yue, Y. et al. (2022) Nature structural & molecular biology 29, 688-697. PMID: 35817871

4. Wang, WW. et al. (2024) Cell 187, 1460-1475.e20. PMID: 38428423

Note

For research use only.

Images

References

Datasheet

Anti-APLN Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-APLN Polyclonal Antibody (HV404014)

Overview

Images

References

Datasheet

SDS

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