Anti-APPL1 Polyclonal Antibody (HV723014)

Name: Anti-APPL1 Polyclonal Antibody
Brand: abinScience
SKU: HV723014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HV723014

Description

Anti-APPL1 Polyclonal Antibody (HV723014) is a rabbit polyclonal antibody detecting APPL1 in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human APPL1 (Phe404-Ser673).

Target

APPL1, Dip13-alpha, APPL, DIP13A, DCC-interacting protein 13-alpha, KIAA1428, Adapter protein containing PH domain, PTB domain and leucine zipper motif 1

Purification

Purified by antigen affinity column.

Accession

Q9UKG1

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

DCC-interacting protein 13-alpha (APPL1) is a ~79 kDa protein. Multifunctional adapter protein that binds to various membrane receptors, nuclear factors and signaling proteins to regulate many processes, such as cell proliferation, immune response, endosomal trafficking and cell metabolism. Regulates signaling pathway leading to cell proliferation through interaction with RAB5A and subunits of the NuRD/MeCP1 complex. Functions as a positive regulator of innate immune response via activation of AKT1 signaling pathway by forming a complex with APPL1 and PIK3R1. Inhibits Fc-gamma receptor-mediated phagocytosis through PI3K/Akt signaling in macrophages. Regulates TLR4 signaling in activated macrophages.

1. Mitsuuchi, Y. et al. (1999) Oncogene 18, 4891-8. PMID: 10490823

2. Miaczynska, M. et al. (2004) Cell 116, 445-56. PMID: 15016378

3. Wang, C. et al. (2009) The Journal of biological chemistry 284, 31608-15. PMID: 19661063

4. Prudente, S. et al. (2015) American journal of human genetics 97, 177-85. PMID: 26073777

5. Song, J. et al. (2016) Oncotarget 7, 279-92. PMID: 26583432

6. Cheng, KK. et al. (2014) Diabetes 63, 3748-58. PMID: 24879834

Note

For research use only.

Images

References

Datasheet

Anti-APPL1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-APPL1 Polyclonal Antibody (HV723014)

Overview

Images

References

Datasheet

SDS

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